Use of Reversed-phase HPLC (RP-HPLC)

Use of Reversed-phase HPLC (RP-HPLC) Introduction Reversed-phase HPLC (RP-HPLC) is one of the commonly used techniques in separation of a wide ranging of analyte based on differences in their structures. In RP-HPLC, the polar mobile phase and nonpolar stationary phase was practised. In this mode of HPLC, the analyte retains into stationary phase by hydrophobic interaction (Hanai, 1999; Swadesh, 2001). The surface area of nonpolar part of analyte molecules is more readily to bind to the RP-HPLC stationary phase. Thus, the less polar analyte will has longer retention time, whereas polar analyte elute more readily. Stationary phase and Column packing In RP-HPLC, the stationary phase is employing of alkylated silica gel column. In other words, the column is packing of nonpolar hydrophobic organic species (eg. octyl, octadecyl and phenyl groups). These hydrophobic organic species attached by siloxane bonds (-Si-O-Si-) to the silica surface (Corradini, Katz, Eksteen, Schoenmakers, Miller, 1998). C18 and C8 phase are most commonly used in the column of RP-HPLC. C18 phase is more hydrophobic as it has longer alkyl chain length which bonded to silica gel. C8 has shorter alkyl chain length compared to C18. It has less carbon attached on silica gel. Besides, phenyl group that bonded to silica surface develops the interaction of aromatic ring (Waksmundzka-Hajnos Sherma, 2011). Mobile Phase The organic solvent such as methanol and acetonitrile are most widely used as mobile phase in RP-HPLC. It is due to they have the characteristic of free of particles and UV transparency (Swadesh, 2001). In addition, acetonitrile, methanol and tetrahydrofuran also served as organic modifiers in RP-HPLC. According to Hanai(1999), the concentration of organic modifiers could alter the overall retention time of analytes. However, the relative changes in retention time are still depends on the properties of analytes (Hanai, 1999; Waksmundzka-Hajnos Sherma, 2011). Furthermore, isocratic and gradient elution also applied in RP-HPLC. In isocratic elution, mobile phase composition is remained constant throughout the elution. For gradient elution, the mobile phase composition is changed continuously throughout the elution. Gradient elution gives a better separation peaks for early eluters and sharper peaks for late eluters, but it need greater skills for method development (Ahuja Dong, 2005) . Detector There are many different spectrophotometric detectors that can be used in RP-HPLC. The main function of the detector in RP-HPLC is to trace and detect the presence of analyte components in the chromatography medium. These detectors are including ultraviolet-visible(UV-VIS) detector, diode array detector(DAD), chemiluminescence nitrogen detector(CLND), refractive index(RI) detector, mass spectroscopy(MS) and others (Swadesh, 2001). UV-VIS detector is the most popular detector among spectrophotometric detectors. Waksmundzka-Hajnos Sherma (2011) stated that UV-VIS detector is utilized by referring to interaction of electromagnetic radiation with analyte sample at the wavelength in the region of 190nm to 1100nm. A high sensitivity of detector is crucial as it also control the signal noise level and drift of the baseline. Applications RP-HPLC methods mostly used to detect known or unknown substances in sample for the quality control. It is applicable in food chemistry, forensic chemistry, pharmaceutical chemistry, toxicological analyses, herbal drugs analyses and others. 2.0 Determination of drug samples by RP-HPLC Reversed-phase High Performance Liquid Chromatography (RP-HPLC) has been generally and effectively utilised to determine numerous drugs samples. Dual wavelength detectors have been employed in the simultaneous investigation of two antispasmodic drugs, phloroglucinol (PG) and its methylated derivative tri-O-methylphloroglucinol (TMP), that are established as pain-relieving drugs when used in combination as they manage to suppress the catechol-O-methyl transferase, relax the smooth muscles, and decrease the abdominal pain induced by glycerol. Present method employing RP-HPLC successfully separate and quantify PG and TMP by using isocratic elution and dual wavelength technique. Apart from studying the injectable sample solution, the serum extracted from blood of healthy volunteers and degraded compounds have also been investigated in order to obtain a complete analysis. Plasma was first centrifuged out of the blood, deproteinated and stock sample was added into the serum obtained. Degrading agent and stress conditions of hydrolysis were used in aid to degrade the PG and TMP. It has been investigated that only oxidation degraded the molecule drastically. (Hasan et al., 2013) Cinitapride hydrogen tartarate has been also estimated by RP-HPLC. Cinitapride hydrogen tartarate is a new prokinetic drug, used as antiulcer agent of the benzamide by acting as an agonist of the 5-HT1 and 5-HT4 receptors and antagonist for 5-HT2 receptors. The preparation of sample solution for this work is simple, by just powdered the tablets and mixed with the mobile phase used. The retention time obtained for cinitapride hydrogen tartarate is 3.737 min. Method validation and optimization have been carried out to validate the findings. (Reddy, Shekar Murali, 2012) Simultaneous investigation of lisinopril and non-steroidal anti-inflammatory drugs (NSAIDs) has been employed in bulk, pharmaceuticals formulations and human serum by RP-HPLC. The major uses of lisinopril are treating hypertension and congestive heart failure, preventing renal complications caused by diabetes while NSAIDs (naproxen, flurbiprofen, diclofenac sodium and mefenamic acid) are primarily used for treatment of acute or chronic pain and inflammation. As there might be interactions between lisinopril and NSAIDs, these two compounds must be detected simultaneously. (Sultana, Arayne, Siddiqui Naveed, 2012) Paracetamol, grouped in non-steroidal anti-inflammatory drugs has been discovered by colorimetric and spectroflurimetric techniques and being realised that it can combine with other drugs and thus, determined singly by RP-HPLC. It has antipyretic, analgesic and weak anti-inflammatory action, generally administered to suppress violent pains in advanced cancers. It has been found out that as concentration of one of mobile phases, ACN is high, paracetamol undergone improper dissolution. Meanwhile, phenomena such as broadening, fronting and tailing were remarkably lessened as ACN concentration gradually decreases. (Devi et al., 2013) Due to the rapid growth of demanding of NSAIDs, it is essential that to determine not the NSAIDs only, but also their combination drugs. The main function of NSAIDs is said to be inhibited cyclooxygenase in vitro and in vivo, hence decreasing the synthesis of prostaglandins which mediate the inflammation. Fifteen drugs have been simultaneously examined by robustness approach, including aceclofenac (ACF), aspirin (ASP), diclofenac (DCF), etoricoxib (ETC), ketorolac (KTL), paracetamol (PCM), salicylic acid (SA), ibuprofen (IBF) and naproxen (NPX) while the combination drugs being studied are clopidogrel (CLP), thiocolchicoside (THC), dextromethorphan (DXM), moxifloxacin (MXF), chlorpheniramine maleate (CPM) and domperidone (DOM). By varying the method parameters, effect on chromatographic separation of all the drugs can be investigated. (Patel, Samanthulam Shrigod, Modh Chaudhari, 2013) Olmesartan medoxomil is an effective antihypertensive reagent, functioned as inhibitor that prevents the angiotensin II from binding to the AT1 receptors in vascular muscle. The validated analytical method for the Olmesartan medoxomil determination in the presence of its degraded product in bulk drug has been established. The degraded products are formed under the conditions suggested by International Conference of Harmonization (ICH), which are acid hydrolysis (0.1M HCL), alkaline hydrolysis (0.1M NaOH), oxidation (30% H2O2), photolysis (UV), and thermal degradation under stress conditions. The sample was found to be highly susceptible to acid and alkaline hydrolysis and oxidation while for other conditions, no degradation was performed. (Hamrapurkar Gadapayale, 2013) Quantitative determination of oseltamivir phosphate (OSP) has been exercised by RP-HPLC. OSP is the drug to treat swine flu, prevents the virus from releasing by infected cells by selectively blocks the viral surface enzyme neuraminidase. Oseltamivir phosphate is also the drug of choice for treatment of avian influenza that diagnosed to be caused by H1N1 virus. This quantitative method was statistically validated for linearity, precision, accuracy, ruggedness, robustness and sensitivity. (Malipatil, Jahan Patil, 2011) 3.0 Determination of food samples by RP-HPLC Reversed-phase High Performance Liquid Chromatography (RP-HPLC) has been widely used to determine the food samples. Honey is a food sources which consists of supersaturated solution of sugars (mostly fructose and sucrose) and other significant amount of minor compounds such as organic acids, furanic aldehydes and acids, enzymes, amino acids and proteins, mineral and water-soluble vitamins. The characterization of these minor compounds have been known to be a reliable tool to determine the botanical an geographical origin as well as the quality of the honey. Many methods have been launched out for the determination the minority organic compounds such as furanic aldehydes and acids but less for vitamins in honey. So, a new analytical method was proposed which utilize RP-HPLC to determine the presence of five water-soluble vitamins in honey that are vitamin B2 (riboflavin), vitamin B3 (nicotinic acid), vitamin B5 (pantothenic acid), vitamin B9 (folic acid) and vitamin C (ascorbic acid). Variety of validation parameters have been carried out in this proposed method in term of detection and quantificat ion limit, linearity, precision, sensitivity as well as the bias to validate their findings (Ciululu et al., 2011). In fact, due to the higher concentrations of saccharides, slightly acidic condition and water activity as well as the presence of organic acids in honey, it favor the formation of furanic aldehydes especially 5-hydroxymethyl-2-furaldehyde (HMF). Hence, HMF is a good parameter for determine the quality of the honey. Apart from this, 2-furaldehyde, 2-furoic acid, 3-furaldehyde and 3-furoic acid also has been quantified in honey samples. Hence, 5-hydroxymethyl-2-furaldehyde (HMF), 2-furaldehyde (2-F), 3-furaldehyde (3-F), 2-furoic acid (2-FA) and 3-furoic acid (3-FA) has been investigated simultaneously and completely validated on 18 honey samples which different in their age, botanical and geographical origin by RP-HPLC. From the result, HMF was quantified in all samples. 2-F and 2-FA was showed in almost half of the samples whereas 3-F was detected in three honey samples and 3-FA in only one. Validation parameters were performed in term of detection limits, precision, linearity and ac curacy (Spano et al., 2008). Brominated phenols have been known to have strong odor properties and act as key flavor compounds in seafoods. The presence of the bromophenol compounds, their concentration and the marine environments controlled the different in the strength and characteristic of odor and flavor in seafoods. Hence, RP-HPLC was employed to determine the presence of simple bromophenols in marine fishes simultaneously which include 2-bromophenol (2-BP), 4-bromophenol (4-BP), 2,4-dibromophenol (2,4-DBP), 2,6-dibormophenol (2,6-DBP) and 2,4,6-tribromophenol (2,4,6-TBP). All the bromphenols have been extracted from the fish samples by combined steam distillation-solvent extraction (SDE) with 2mL of pentane/diethyl ether (6:4) and identified by RP-HPLC with UV-detection. (Silva et al., 2005) Nowadays, the employment of food additives in the production of processed and fast foods was gradually increasing throughout the world. However, illegal and excess addition of food additives can cause significant health problems were well known by community. For an example, in china, the ministry of Health of the People’s Republic of China strongly prohibited the addition of acesulfame, saccharin, neotame, stevioside, benzoic acid, caffeine and dehydroacetic acid as well as regulated the addition level of sorbic acid to 0.2g/kg into red wine. Variety of methods is available to determine the food additives present in food and drinks. However, simultaneous determination of large amount of food additives in red wine which mentioned as above was proposed by using dispersive solid phase extraction (dSPE) followed by RP-HPLC with ultraviolet (UV) detection in this study. The red wine samples were undergoes the dSPE methods which considered as most powerful cleanup technologies in re moving various matrix and then analyzed by RP-HPLC with UV detection. Different kinds of validation parameters were utilized to determine the satisfaction and accuracy of the results. Apart from this, amino-functionalized Fe3O4 magnetic polymer (MP) which coupled with tetraethylenepentamine (TEPA) also proved that it is an efficient absorbent in dSPE extraction procedure which could eliminate most of the interferences in the red wine. (Zhao et al., 2013) Apart from this, the dosage of additives especially preservatives, is the most strictly controlled by EU law because of their potential risk to human health and safety. Nationally and international authorities have been established the guidelines for the usage of preservatives in food and foodstuff which about the maximum dosage, use conditions and type of food in which they can be used. In our daily life, the most common preservatives are sorbic acid, natamycin and lysozyme. So, analytical method for simultaneous determination of the four preservatives in different kinds of cheeses that are sorbic acid, natamycin, lysozyme and benzoic acid by single RP-HPLC was established. Benzoic acid was also included into the determination step is because it can be synthesized naturally from the microbial metabolism even it’s not added technically to cheese during production. All preservatives were extracted from the samples through a simple extraction step and then separated by RP-HPLC. Finally, the analytes were analyzed by a single wavelength UV detector (280nm) and even a triple wavelength UV detector (227nm, 280nm and 303nm) for a more sensitive determination. (Guarino et al., 2011)

Article 62 of the Vienna Convention Essay

The doctrine of fundamental change of circumstances, (rebus sic stantibus) is a principle of customary international law allowing a part to an agreement to withdraw or terminate it where there has been a fundamental change in circumstances. The main justification for this principle is that treaties often remain in force for many years, during which time fundamental changes may occur in the political or international environment which may require a departure from the provisions set out in the treaty. However, this principle has also been criticized for disrupting the binding force of obligations taken by states, particularly when bearing in mind that there is no compulsory jurisdiction forcing states who terminate a treaty on this ground, to have the legitimacy of their decision scrutinised before an international tribunal. Nevertheless, this principle has been codified in Article 62 of the VCLT, although its scope has been severely restricted. Article 62 (1) is drafted it negative terms, stating that a fundamental change of circumstances which has occurred since the conclusion of a treaty, and which was not foreseen by the parties, may not be invoked as a ground for terminating or withdrawing from the treaty unless: (a) the existence of those circumstances constituted an essential basis of the consent of the parties to be bound by the treaty; and (b) the effect of the change is radically to transform the extent of obligations still to be performed under the treaty. This is further restricted by Article 62 (2) which states that fundamental change of circumstances may not be invoked as a ground for terminating or withdrawing from a treaty if the treaty establishes a boundary or if the fundamental change is the result of a breach by the party invoking it either of an obligation under the treaty or of any other international obligation owed to any other party to the treaty. If these conditions are met, Article 62 (3) allows a state to suspend the operation of the treaty if it does not wish to terminate it or withdraw from  it. Therefore, in order for Article 62 to apply, five conditions must be met. First, the change must be of circumstances existing at the time the treaty was made. Second, the change of circumstances must be â€Å"fundamental†. Third, the change must not have been foreseen by the parties. Fourth, the existence of those circumstances must have constituted an essential basis of the consent of the parties to be bound by the treaty in the first place. Fifth, the effect of the change must be radically to transform the â€Å"extent† of obligations still to be performed under the treaty. The possibility of terminating a treaty on grounds of fundamental change of circumstances was recognised in the Fisheries Jurisdiction case, but was not held to be applicable on the particular facts of that case. In this case, Iceland and the UK entered into an agreement to limit their fisheries jurisdiction to within their 12 mile continental shelf limit. However, as the law of the sea developed, greater fishing zones became permissible, and Iceland argued that these developments where a fundamental change of circumstances allowing them to withdraw from the treaty. The ICJ held that a change in the law is not sufficient to constitute a fundamental change of circumstance. A change in international law could however terminate the treaty on grounds of supervening impossibility of performance if the change made the carrying out of the treaty illegal. It was stated that changes in circumstances will only be regarded as fundamental if they â€Å"imperil the existence or vital development of one of the parties.† Moreover, it was added that â€Å"the change must have increased the burden of the obligations to be executed to the extent of rendering the performance something essentially different from that initially undertaken†. As these requirements had not been met, Iceland could not withdraw from the agreement. Similarly, the scope and application of Article 62 was also examined in the Gabcikovo-Nagymaros Project (Danube Dam) case. Here the ICJ stated that â€Å"The changed circumstances advanced by Hungary are, in the Court’s view, not of such a nature†¦that their effect would radically transform the extent of the obligations still to be performed in order to accomplish the Project. A  fundamental change of circumstances must have been unforeseen; the existence of the circumstances at the time of the Treaty’s conclusion must have constituted an essential basis of the consent of the parties to be bound by the Treaty. The negative and conditional wording of Article 62 of the Vienna Convention on the Law of Treaties is a clear indication moreover that the stability of treaty relations requires that the plea of fundamental change of circumstances be applied only in exceptional cases†. This reasoning was followed by the ECJ in the Racke v Hauptzollamt Mainz case. In this case, the ECJ considered whether the EC could suspend the operation of a trade concession agreement between itself and the former Republic of Yugoslavia on the grounds that the break-up of Yugoslavia into several new States were factors to be regarded as a political change, involving a fundamental change in the material circumstances underlying the consent of the contracting parties bound by the agreement. It was held not to be, because the change did not fundamentally alter the extent of the obligations under the agreement, which was essentially an economic agreement to promote the development of trade between the contracting parties. These cases demonstrate the severe restriction which is place on the scope of Article 62, and that it will require a truly exceptional case for the ICJ to conclude that the requirements of Article 62 have been met. In relation to the provisions of Article 62, a question has arisen as to whether these provisions could apply to the Anti-Ballistic Missile (ABM) Treaty between the USA and the USSR in 1972, which was concluded in the circumstances of the Cold War. Can it be said that there has been a fundamental change in the circumstances which determined the parties to accept the treaty and which has resulted in a radical transformation in the extent of their obligations still to be performed under the treaty? In 2001, President Bush announced that he had given formal notice to Russia, that the US is withdrawing from the Treaty. The treaty sought to limit the development and deployment of nationwide ballistic missile defence systems. Explaining the withdrawal, President Bush referred to the terrorist events  of September 11 as a fundamental change of circumstances which have resulted in the US now being put in danger from rogue states or terrorists who may have acquired nuclear weapons. Consequently, US wants the freedom to develop effective defenses against missile attacks from such sources, and this can be done only if the restrictions of the ABM Treaty are removed. A further argument that can be made is that the disintegration of the Soviet Union and the removal of the Cold War threat may also constitute a fundamental change in circumstances allowing the US to withdraw from the treaty. However, the problem here is that despite the end of the Cold War and the disintegration of the Soviet Union, both the US and Russia have continued to regard the treaty as remaining in force between themselves. The most obvious resolution to this problem would be for Russia to grant its consent to the US to withdraw from the treaty, or for both states to terminate the treaty by mutual agreement, according to Article 54 of the VCLT. However, it is unlikely that Russia will grant its consent for the USA to withdraw from the treaty. Therefore, the question of whether the US can unilaterally withdraw from the treaty arises. The only provision under the VCLT which may provide the US with the opportunity to unilaterally withdraw from the treaty is fundamental change of circumstances, under Article 62. In order for Article 62 to apply, five conditions must be met. First, the change must be of circumstances existing at the time the treaty was made. The first condition is clearly met as there certainly has been a change of circumstances from the height of the cold war in 1972 to the present post-cold war era. The Cold War has now subsided, and the Iron Curtain and the Soviet Union no longer exist. Second, the change of circumstances must be â€Å"fundamental†. This change can be said to be fundamental because in 1972, the threat from the Soviet Union was real and vivid, whereas the improvement in relations since then has greatly reduced this threat. This disparity between US and Russian military power  can be seen by the fact that the US spends more on its military than the next ten military powers (including Russia) taken together. Furthermore, few countries other than the US and Russia had nuclear weapons in 1972, and the majority that did were allies of the US. Any threat from other countries (let alone terrorists) was mostly a distant threat, not yet a reality. Third, the change must not have been foreseen by the parties. It is questionable whether the change in circumstances was not foreseen by the parties. It can be argued that the acquisition of nuclear weapons by other countries, some of which are a threat to the US was foreseeable. However, the threat from terrorists who may have now acquired nuclear weapons cannot reasonably be said to have been foreseeable. Fourth, the existence of those circumstances must have constituted an essential basis of the consent of the parties to be bound by the treaty in the first place. It can be said that the circumstances which constituted an essential basis of the US’s consent have changed, because the Soviet Union no longer exists, and Russia is no longer the threat it used to be. The only reason why the US signed the treaty was because of the threat from Russia, if there was no threat, it would not have consented. Furthermore, the persisting ideology of the two states at the time the treaty was signed was world supremacy, and so both states sought to limit the military and nuclear aspirations of the other by consenting to this treaty. It would now seem that the original nuclear arms race which instigated the treaty is over, since neither the US nor Russia are seeking to increase their arsenal of nuclear weapons, and it is doubted whether Russia would have the financial means to do so even if it wanted to. Therefore, it seems that the object and purpose of the ABM Treaty has ceased to be relevant in today’s world. On the other hand, it may be argued that despite the developments of the last 30 years, Russia has still not submitted to the emerging realisation that we are living in a US dominated world. It continues to maintain links with rogue governments and has nuclear parity with the US. Therefore, although it  is less of a threat than it used to be, it is still nevertheless a threat. Consequently, the counter-argument may be that the original threat which instigated the US to sign the ABM Treaty still exists, albeit in a weaker form. Therefore, it is a matter of debate whether the existence of the circumstances which initially formed the basis of the US’s consent have been fundamentally changed. Fifth, the effect of the change must be radically to transform the â€Å"extent† of obligations still to be performed under the treaty. This is the most difficult condition to satisfy and it is questionable whether it has been satisfied. It must be remembered that both the US and Russia have continued to regard the treaty as remaining in force between themselves, and the treaty has continued to carry out its purpose, i.e. the restriction of nuclear weapons. However, the justification given by the US is that the Treaty enshrines the past and should not prevent it from addressing the threats of today, or to pursue technology to defend itself. This argument may have some support from the statements made in the Fisheries Jurisdiction case, where it was stated that changes in circumstances will only be regarded as fundamental if they â€Å"imperil the existence or vital development of one of the parties.† Moreover, it was added that â€Å"the change must have increased the burden of the obligations to be executed to the extent of rendering the performance something essentially different from that initially undertaken†. It can be argued that preventing the US from developing technology to protect itself from the threat from rogue nations and terrorists may indeed â€Å"imperil its existence or vital development†. Moreover, as Professor Mullerson points out, the purpose of the ABM Treaty and the extent of its obligations were not to prohibit national missile defence systems in abstract against all possible threats, but to prohibit such defence from the other party to the Treaty. Therefore, by preventing the US from defending itself from new nuclear threats, it can be said that the fundamental change has now altered the extent of the obligations of the US. This is because its obligations will have been transformed from an obligation not to defend itself against Soviet missile threats, to an  obligation not to defend itself from missile threats from rogue states or terrorists. Therefore, the fundamental change of circumstance provision in Article 62, when combined with the provision in Article XV of the ABM treaty itself which allows a state to withdraw where extraordinary events arise, and where its supreme interests are threatened, may give the US a legitimate basis for unilaterally withdrawing from the treaty. As Professor Mullerson points out, â€Å"it is clear that US-Soviet (Russian) legal relations cannot, by definition, remain the same as they were at the height of the military-strategic competition which was the axis of world politics†. On the other hand, it may be argued that despite the developments of the last 30 years, although Russia is less of a threat than it used to be, it is still nevertheless a threat. Consequently, the counter-argument may be that the original threat which instigated the US to sign the ABM Treaty still exists, albeit in a weaker form. An alternative solution to the situation is advanced by Professor Mullerson, who states that the doctrine of change of fundamental circumstances should be used not only as a method of upholding or terminating treaties, but it should be used more flexibly as a process for initiating renegotiation or modification of a treaty whose object and purpose no longer correspond to the needs of changed circumstances. Despite the arguments either way, if Russia ultimately acquiesces in the US justification for withdrawing from the ABM Treaty, it is possible that the reasons given by the US may be regarded as supplying a precedent for withdrawal by the United States or other countries from other arms control treaties on similar grounds. As we have seen from the above discussion, although Article 62 lays down the general criteria which may allow a state to terminate a treaty on grounds of fundamental change of circumstances, the scope and application of Article 62 remains very problematic. The fact that there is no compulsory jurisdiction forcing states who terminate a treaty on this ground, to have the legitimacy  of their decision scrutinised before an international tribunal further adds to these problems. In the few cases where Article 62 has been ar5gued before the ICJ, we have seen that a very strict approach has been adopted and that no state has ever successfully pleaded Article 62 before the ICJ. This may discourage states from testing the legitimacy of their decision to terminate a treaty on the basis of Article 62 before the ICJ, as will probably occur in the ABM Treaty debate. Perhaps an alternative solution would be to not only use Article 62 as a method of upholding or terminating treaties; but more flexibly as a process for initiating renegotiation or modification of a treaty whose object and purpose no longer correspond to the needs of changed circumstances.

The American Way: Courage, Pride, and Honor

The American Way: Courage, Pride, and Honor â€Å"These are the times that try men’s souls. The summer soldier and the sunshine patriot will, in this crisis, shrink from the service of their country: but he that stands it now, deserves the love and thanks of man and woman. † These words, uttered by the great Thomas Paine on December 23, 1776, embody the true courage and sacrifice of the many American Patriots during the earliest stages of our country. It is words and ideals such as these that shaped and molded our country into the place we know it as today.As both an American citizen and student of US history, I believe that a man’s true character reveals itself in times of adversity. The leaders emerge and men and women of smaller fortitude weed themselves out, sometimes sooner rather than later. The early stages of our country were marked with great struggle and rebellion against the powers that kept us down. There has been struggle throughout the entire histor y of our country, and still is today, but not many quite like our struggle to gain independence and freedom.Our country made its first true attempt to gain independence from King George the III by declaring ourselves an independent nation free from any bondage to Great Britain. This led to the Revolutionary War, in which over 4,000 men gave their lives and another 6,000 were wounded, in the attempt to gain freedom for all Americans. General George S. Patton later explained what drove these men to go to any length for their country when he said, â€Å"The courageous man is the man who forces himself, in spite of his fear, to carry on.Discipline, pride, self-respect, self-confidence, and the love of glory are attributes which will make a man courageous even when he is afraid. † Thousands of men have died throughout the history of this country so that we may be able to live the life we live today, with nobody ruling us and the freedom to worship as we please, live how we please, and aspire to make the most out of ourselves in every aspect of life. Our country takes pride in the men and women who have given the ultimate sacrifice for their country, and hopefully we shall never be so foolish to forget the sacrifices made.We fly our flags at full height to remember these men, we celebrate independence day, and we celebrate veterans day because we need to remember the violence and adversity in order to appreciate the peaceful and prosperous lives we are able to live today. I’ve already spoken about the freedom fighters in the beginning of our time as a nation. However, many others have fought for freedom and equality in order to shape our nation into what it is today. The African-Americans had to fight in the 1960’s for equality.Imagine coming to Lackawanna College and whites and blacks drinking from separate water fountains and eating in different cafeterias. This would truly be a sad representation of the character of the average American man. Even before this the Irish immigrants were hated and discriminated against, but also the Italian and Polish immigrants. So many people have struggled and persevered for the better of our nation. I believe the American experience is one of courage, hardship, perseverance, freedom, and equality for any man who chooses to come here and pursue these ideals.John F. Kennedy described perfectly the importance of these beliefs and standards when he said, â€Å"Let every nation know, whether it wishes us well or ill, that we shall pay any price, bear an burden, meet any hardship, support any friend, oppose any foe, in order to assure the survival and success of liberty†¦ We dare not tempt them with weakness. For only when our arms are sufficient beyond doubt can we be certain beyond doubt that they will never be employed. †

Ethics Is The Diagnostic Process For Many Reasons - 983 Words

It comes to no surprise that ethics governs the diagnostic process for several reasons. To diagnose a client means to define in professional terms the nature, boundaries, and intensity of a problem a client brings to counseling. In order to achieve diagnosing clients in the most efficient manner, one must use a procedure referred to as an assessment. There are several different assessment procedures that have been developed to help assess diagnosis. Assessments are articulated in a variety of ways; and the more accurate the assessment of the problems, the more likely those problems will be successfully resolved. According to Welfel, â€Å"we overall cannot treat what we have not identified† (Welfel, 2015). I have created a case in which I am the professional being asked to conduct assessments on a client. However, I receive several calls asking for the results of the assessment. The callers included are: the client’s attorney, the opposing attorney, the client himself o r herself, the client’s parents, the client’s employer, and the judge trying a case in which the client is a defendant. In regards to who I can legally and ethically give the results to, I must be cautious of my client’s welfare, but still aware of what I am required to give. Modifications in state and federal regulations have significantly changed the contents of the ethics codes in recent years regarding test security. The regulations require that clients are allowed to view their treatment records, asShow MoreRelatedWhat I Be A Doctor817 Words   |  4 Pageshumans, they are so complex yet so simple. And somehow this paradox continues when it comes to biology - human body, so complex yet so simple. It intrigues me, fascinates me and ignites a passion inside me to understand it better. And that is the reason I have chosen internal medicine as my career for life. By the way, in first few lines, ‘he’ refers to a beloved teacher. Being from a small town where everybody knew everybody, our interactions were not limited to school. More often than not, weRead MoreEthics : Ethics And Ethics888 Words   |  4 PagesEthics Ethics is a branch of philosophy that involves an organize system, defending, and recommending concepts of right and wrong behavior. Ethics researches the two questions What is the best way for people to live? and What actions are right or wrong in particular circumstances? Ethics attempts to settle questions of moral behavior by defining what is good or bad and right or wrong. There are three branches in ethics, meta-ethics, normative ethics, and applied ethics. Meta-ethics deals withRead MoreGeneral Electric Medical Systems - Global Product Company concept1028 Words   |  5 Pagesstandards most cost-effectively†. That means that the production is moving to countries where people are mostly underutilized (the example given in the case study tells about engineers from Eastern Europe, who cost only $1,5/h). There are two major reasons why a company would do that: a. Maintaining/Raising the margins - One of the most important goals of every company is profit. It can be raised in two ways: either a company has to make bigger revenues or it has to lower costs. When a company canRead MoreMental Health Case Study1499 Words   |  6 PagesThere are many mental health facilities around the state and the country, being able to attend and observed in one was very difficult because of confidentially purposes. Many agencies I visited such as Catholic Charities, St. Mary Hospital behavioral health services, covenant house had turned me down not allowing me to have that exposure and visit in their organization. The agencies policies were strict, they had mentioned they will have to do a series of background checks and had to be enroll inRead MoreThe Space Shuttle Disaster And 121350 Words   |  6 Pagesto come with many new ideas, experiments to try and space to explore. While this might have been the case for a few years following, NASA couldn’t have predicted what was to happen on the mornings of January 28, 1986 and February 1, 2003†¦ or could they? Disaster struck for both the Challenger and the Columbus space shuttle s. Even though both scenarios were different, they were one in the same when it came to the reasoning behind why it happened. The ideas of cultural change, ethics of organizationRead MoreThe Nurse As A Nurse919 Words   |  4 PagesMany years ago, the nurse job was viewed merely as the bedside care nurse, the physician assistant. If you want to be a nurse, you need to feel the passion for helping others because the core of nursing is caring. Nowadays, the nursing profession and the role of the nurse as a caregiver is broad, with a holistic approach; the nurse is now an educated nurse, autonomous, and responsible in the promotion of quality care. The nursing profession has developed along with the social, economic and technologicalRead MoreNarrative Summary : Nurses Nurse Manager1654 Words   |  7 Pagesrole for the past 5 months and has made changes and improvements for the betterment of the service and at the organizational level. A. Practice Practice: Mr. Thathamkulam provides leadership in the application of the nursing process to patient care, organizational process at different levels, including programs and service levels. This is evident in his day to day initiatives in taking care of the patients as well as guiding his staff in the clinic to make patient care and flow efficient. In additionRead MoreEthical Nursing1709 Words   |  7 PagesThe aim of this essay is to discuss an ethical issue encountered whilst on placement. The discussion will focus on the influence of the key ethics theories of deontology and utilitarianism, ethics principles of autonomy, beneficence, non-maleficence and justice on decision-making in practice. It will consider the patients’ and staffs’ values and beliefs, legislation and professional practice. Confidentiality will be respected by using a pseudonym, Carol, in accordance with the Nursing and MidwiferyRead MoreAssisted Suicide Essay1534 Words   |  7 Pageswhich is the process of ending their life with help from another person. Assisted suicide, despite the chronic or degenerative illness, has caused great controversy. The debate arises when the question about whether or not the practice should be legalized is asked. Some say that every person has a God given right to do what they want with their bodies, when they want to do it. Others say that palliative staffs should be focusing on studying better ways to eliminate pain and make the process of passingRead MoreBoundaries Violation Dual Relationships Essay1443 Words   |  6 Pageswith client clarifying reason for discontinue treatment being based on the best care of the patient, informing he is not abandoning her, according to APA (2010); taking reasonable steps to avoid impairing their clients, students, supervisors, research participants, organization clients, and other with whom they work and to minimize where it is foreseeable terminating and unavoidable† †¦ authorized by law or administr ative guideline or as otherwise as delivered in this Ethics Codes†(sec. 3.10 (a),